elevated-plus maze apparatus  (Panlab)

Journal: bioRxiv

Article Title: Astrocytic RNA degradation suppresses calcium signaling to support synapse function and restrain anxiety

doi: 10.1101/2025.11.18.689044

Figure Lengend Snippet: ( A ) Open field beam breaks display no differences in locomotion amongst CTRL and cKO animals (CTRL: 3892 ± 271; cKO: 4076 ± 239, n = 22 mice per group, p = 0.61, Unpaired t -test). ( B ) Upf2 cKO mice show reduced time in open arms of elevated plus maze indicating anxiety-like behavior (CTRL: 38.5 ± 3.9; cKO: 23.09 ± 2.6, n = 31 mice per group, p = 0.0036, Unpaired t -test). ( C ) Astrocytic Upf2 loss is associated with reduced marble burying (CTRL: 16.3 ± 0.6, n = 22 mice; cKO: 13.0 ± 0.8, n = 23 mice, p = 0.0003, Mann-Whitney test). ( D ) Contextual fear conditioning paradigm revealed differences in contextual memory on Day 2 between CTRL and cKO animals (CTRL: 68.3 ± 3, n = 29 mice; cKO: 77.6 ± 3, n = 24 mice, p = 0.02, Mann-Whitney test). No differences were detected on Day 1 and Day 3 (Day 1: Baseline vs. UC-CS pairings, CTRL vs. cKO, p > 0.59, Sidak’s multiple comparisons test; Day 3: CTRL vs. cKO, p = 0.69, Unpaired t -test). Assessment of baseline freezing revealed Upf2 cKO mice show significant elevation on Day 2 and Day 3 as compared to CTRL (Day 1: CTRL: 1.8 ± 0.6; cKO: 6.1 ± 2.1, p = 0.43, Mann-Whitney test. Day 2: CTRL: 12.4 ± 0.8; cKO: 14.6 ± 0.7, p = 0.048, Unpaired t -test. Day 3: CTRL: 21.2 ± 3.6; cKO: 34.9 ± 4.5, p = 0.03, Mann-Whitney test. CTRL: n = 29 mice, cKO: n = 24 mice). Data are presented as mean ± S.E.M. * denotes p < 0.05, ** denotes p < 0.01, *** denotes p < 0.005, ns = not significant. This figure illustrates that astrocytic UPF2 depletion is associated with avoidance behavior and increased baseline freezing.

Article Snippet: Elevated Plus Maze : The Elevated plus maze apparatus was positioned and set for Noldus Ethovision XT software acquisition.

Techniques: MANN-WHITNEY

Journal: bioRxiv

Article Title: Astrocytic RNA degradation suppresses calcium signaling to support synapse function and restrain anxiety

doi: 10.1101/2025.11.18.689044

Figure Lengend Snippet: ( A ) Scheme for viral delivery and timeline for Ca 2+ measurement experiments. ( B ) CalEx diminished Ca 2+ events to CTRL levels in hippocampus (CTRL: 4.5 ± 1.5, n = 6 mice, 32 ROIs; cKO: 5.1 ± 0.9, n = 8 mice, 68 ROIs; cKO + CalEx: 1.4 ± 0.1, n = 4 mice, 46 ROIs, CTRL vs. cKO + CalEx, p = 0.09, Tukey’s multiple comparisons test). CalEx Ca 2+ events are comparable to CTRL in visual cortex (CTRL: 4.9 ± 1.7, n = 4 mice, 16 ROIs; cKO: 6.6 ± 2.1, n = 6 mice, 36 ROIs; cKO + CalEx: 2.3 ± 0.4, n = 4 mice, 19 ROIs; CTRL vs. cKO + CalEx, p = 0.71, Tukey’s multiple comparisons test). CalEx prefrontal cortex Ca 2+ events are similar to CTRL (CTRL: 0.8 ± 0.2, n = 4 mice, 15 ROIs; cKO: 2.3 ± 0.4, n = 4 mice, 20 ROIs; cKO + CalEx: 1.4 ± 0.3, n = 4 mice, 16 ROIs; CTRL vs. cKO + CalEx, p = 0.54, Tukey’s multiple comparisons test). ( C ) Scheme for viral delivery and tamoxifen treatment followed by rescue assays for PSD-95 density, neurotransmission, and animal behavior. ( D ) Total PSD-95 density is not restored to CTRL levels by CalEx manipulation in Upf2 cKO mice (CTRL: 1648 ± 188, n = 24 ROIs, 4 mice; cKO: 459 ± 41, n = 21 ROIs, 4 mice; cKO + CalEx: 771 ± 52, n = 24 ROIs, 4 mice; CTRL vs. cKO + CalEx, p < 0.0001, Tukey’s multiple comparisons test). ( E ) CalEx elevates synaptic strength in hippocampus of Upf2 cKO mice as compared to CTRL (CTRL: Linear fit 1.88 ± 0.1, r = 0.99, n = 6 slices, 3 mice; cKO: Linear fit 0.84 ± 0.05, r = 0.99, n = 5 slices, 4 mice; cKO + CalEx: Linear fit 2.66 ± 0.24, r = 0.98, n = 5 slices, 3 mice. Linear fit slope values: CTRL vs. cKO + CalEx, p = 0.03, Tukey’s multiple comparisons test). ( F ) CalEx increases elevated plus maze open arm time in Upf2 cKO mice (CTRL: 70.7 ± 5.7, n = 11 mice; cKO: 25.9 ± 5.2, n = 8 mice; cKO + CalEx: 49.1 ± 8.1, n = 10 mice; CTRL + CalEx: 40.6 ± 6.4, n = 8 mice, CTRL vs. cKO + CalEx, p = 0.08, Tukey’s multiple comparisons test). Data are presented as mean ± S.E.M. * denotes p < 0.05, ** denotes p < 0.01, **** denotes p < 0.0001, ns = not significant. This figure depicts that restoring Ca 2+ levels in astrocytes of Upf2 cKO mice recovers basal

Article Snippet: Elevated Plus Maze : The Elevated plus maze apparatus was positioned and set for Noldus Ethovision XT software acquisition.

Techniques: